Helen Benitez is an undergraduate student at UCLA majoring in Physiological Sciences and minoring in Global Health. She is a sophomore and a member of the PEERS program. She has been an undergraduate in Dr. Tzung Hsiai’s cardiovascular lab since the spring quarter of her first year. She is currently involved in a zebrafish project involving chemotherapy-induced cardiotoxicity associated with Doxorubicin (Dox). Anthracyclines, such as Dox, are effective chemotherapy drugs widely used to treat various cancers but induce dose-dependent cardiotoxicity, leading to reduced left ventricular ejection fraction and heart failure. The project serves to study the mechano-sensitive pathways involved with anthracycline-induced cardiotoxicity (AIC) to optimize chemotherapy medication usage by introducing a co-treatment with spironolactone (SP). To visualize the induced cardiotoxicity, light-sheet fluorescence microscopy was used to obtain 3D+ time images of the hearts in DMSO control, Dox-treated, SP-treated, and Dox-SP-co-treated embryos. Currently, we continue to analyze bulk-RNA sequencing data to explore underlying genes involved with Dox-induced cardiac dysfunction and SP-reduced cardiac mechanisms. Helen would like to express her immense gratitude for the mentorship and continued support provided by Dr. Hsiai and the rest of the Hsiai lab members. She is sincerely appreciative of the research and career guidance she has received from the Hsiai Lab. She would like to thank the CARE Fellows Program for its generosity in encouraging her research endeavors.